Son of Henry Ambrose of Ormskirk.
Vicar of Ormskirk: instituted 28 Jan. 1537/8; deprived or resigned and succeeded by Richard Ambrose, Feb. 1571/2.
King's chaplain at time of institution. In 1559 he refused to appear at the Visitation.
Bur. at Ormskirk, 1 June 1572.
VCH Lancs., 3.244 and n.; H. Fishwick, The History of the Parish of Garstang, Chetham Soc., 2 vols., vols. 104-5 (1878-9), 2.156-8.